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RNAA01

RNA-Seq Analysis

RNA-Seq analysis training – live online course covering experiment design, data QC, alignment, gene expression, DESeq2 differential expression, PCA, visualisation, and functional analysis.

  • Duration: 4 Days, 3.5 hours per day
  • Next Date: January 26-29, 2026
  • Format: Live Online Format
TIME ZONE

UK (GMT) local time - All sessions will be recorded and made available to ensure accessibility for attendees across different time zones.

£350Registration Fee

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5.0

from 200+ reviews

Course Description

From planning an RNA-Seq experiment to visualising your results and drawing biological conclusions from your data, this workshop will guide you through every step of data analysis. Learn to avoid common pitfalls and fully exploit this powerful technology to delve into the transcriptome of any organism.

What You’ll Learn

By the end of the course participants should:

  • Understand how to design an RNA-Seq experiment
  • Understand the structure of raw RNA-Seq data, and how to perform initial data QC
  • Understand how to align RNA-Seq reads to a genome, and perform post alignment quality assessment
  • Understand how to identify and deal with possible problems in your data
  • Understand the main approaches, and pitfalls in quantifying gene expression
  • Understand principal component analysis, and how this can be used to gain insights into RNA-Seq datasets
  • Understand how to use DESeq2 to perform appropriate differential expression analysis for any experimental design
  • Understand common approaches to visualisation of differential expression analysis
  • Understand functional analysis of differential expression

Course Format

Interactive Learning Format

Each day features a well-balanced combination of lectures and hands-on practical exercises, with dedicated time for discussing participants’ own data, time permitting.

Global Accessibility

All live sessions are recorded and made available on the same day, ensuring accessibility for participants across different time zones.

Collaborative Discussions

Open discussion sessions provide an opportunity for participants to explore specific research questions and engage with instructors and peers.

Comprehensive Course Materials

All code, datasets, and presentation slides used during the course will be shared with participants by the instructor.

Personalized Data Engagement

Participants are encouraged to bring their own data for discussion and practical application during the course.

Post-Course Support

Participants will receive continued support via email for 30 days following the course, along with on-demand access to session recordings for the same period.

Who Should Attend / Intended Audiences

Course is suitable Academics, post-graduate students or biotech employees working on, or planning to work on any type of bulk RNA-Seq data.

Participants should have basic experience of R, RStudio and linux, as well as a basic understanding of transcriptomics and molecular biology.

Equipment and Software requirements

A laptop or desktop computer with a reasonable internet connection and a webrowser. No other software is required.

A working webcam is recommended to support interactive elements of the course. We encourage participants to keep their cameras on during live Zoom sessions to foster a more engaging and collaborative environment.

While not essential, using a large monitor – or ideally a dual-monitor setup – can significantly enhance your learning experience by allowing you to view course materials and work in R or linux simultaneously.

All necessary software is installed already on a virtual machine that you will access through your web browser

Download Zoom

Dr. Frances Turner

Frances is a bioinformatician at Edinburgh University with extensive experience supporting researchers across the life sciences in analysing and interpreting high-throughput sequencing data. Her work focuses on developing and applying bioinformatics pipelines and analytical approaches that enable scientists to extract meaningful biological insights from complex genomic datasets. She works with a wide range of sequencing applications, including whole-genome, transcriptome, and metagenomic data, and has particular expertise in ensuring data quality, reproducibility, and effective downstream analysis.

 

Frances has spent many years collaborating closely with research groups from diverse disciplines, providing both technical expertise and strategic advice on study design, sequencing strategy, and data interpretation. Alongside her consultancy and applied work, she is also committed to training and knowledge exchange, delivering workshops and guidance to help life scientists build confidence in handling and analysing their own sequencing data.

 

Education & Career

  • PhD in Bioinformatics
  • MSc Bioinformatics
  • Bioinformatician at Edinburgh University, providing analysis support and training for high-throughput sequencing projects at the Gene Therapy Mission Hub.

 

Research Focus

Frances’ work centres on enabling researchers to make the most of next-generation sequencing technologies. She is particularly interested in:

  • Developing and refining bioinformatics workflows for diverse sequencing applications
  • Supporting reproducible and scalable approaches to data analysis
  • Bridging the gap between experimental design and downstream interpretation

 

Current Projects

  • Providing end-to-end analysis support for whole-genome, transcriptomic, and metagenomic studies
  • Collaborating with life science researchers on custom data workflows
  • Contributing to training programmes that build bioinformatics capacity in the research community

 

Professional Consultancy

Frances provides expert bioinformatics support to academic and applied research projects, offering guidance on sequencing design, data processing, and statistical interpretation. She regularly collaborates with interdisciplinary teams, ensuring robust, reproducible, and biologically meaningful outcomes.

Teaching & Skills

  • Teaches workshops on sequencing data analysis, quality control, and reproducible pipelines
  • Skilled in a wide range of bioinformatics tools and platforms for genomic and transcriptomic analysis
  • Advocates for open science, reproducibility, and empowering life scientists to engage confidently with their data

 

Links
LinkedIn

Session 1 – 01:00:00 – Introduction to RNA-seq
Understanding of the structure of raw RNA-Seq data how to perform initial data QC

Session 2 – 01:00:00 – RNA-seq data QC
Understanding how to align RNA-Seq reads to a genome, perform post alignment quality assessment, and detect and deal with any problems arising from sample or library preparation.

Session 3 – 01:30:00 – Gene expression quantification
Understanding main approaches to, and possible difficulties in, the quantification of gene expression.

Session 4 – 01:00:00 – Introduction to differential expression analysis and DESeq2
Understanding important principles of differential expression analysis

Session 5 – 01:00:00 – Introduction to PCA analysis
Understanding principal component analysis and (PCA), how to explore RNA-Seq data with PCA, filter samples and merge technical replicates.

Session 6 – 01:30:00 – Introduction to differential expression analysis
Understanding how to use a simple linear model to perform differential expression analysi

Advanced differential expression analysis and visualisation

Session 7 – 01:30:00 – Complex experimental designs
Understanding how to model covariates and continuous variables, and how to make more complex comparisons

Session 8 – 01:00:00 – Review of differential expression analysis workflow
Review of differential expression analysis workflow

Session 9 – 01:00:00 – Understanding methods to visualise expression of genes of interest
Understanding methods to visualise expression of genes of interest

Session 10 – 01:00:00 – Volcano plots and MA plots
Understanding common methods for visualisation of differential expression across a whole data set.

Session 11- 01:30:00 – Functional analysis
Understanding functional analysis of differential expression with FGSEA

Session 12 – 01:00:00 – Discussion of participants projects.
A chance to discuss your own projects and ask more detailed questions relating to your own work

Testimonials

PRStats offers a great lineup of courses on statistical and analytical methods that are super relevant for ecologists and biologists. My lab and I have taken several of their courses—like Bayesian mixing models, time series analysis, and machine/deep learning—and we've found them very informative and directly useful for our work. I often recommend PRStats to my students and colleagues as a great way to brush up on or learn new R-based statistical skills.

Rolando O. Santos

PhD Assistant Professor, Florida International University

Courses attended

SIMM05, IMDL03, ITSA02, GEEE01 and MOVE07

Testimonials

PRStats offers a great lineup of courses on statistical and analytical methods that are super relevant for ecologists and biologists. My lab and I have taken several of their courses—like Bayesian mixing models, time series analysis, and machine/deep learning—and we've found them very informative and directly useful for our work. I often recommend PRStats to my students and colleagues as a great way to brush up on or learn new R-based statistical skills.

Rolando O. Santos

PhD Assistant Professor, Florida International University

Courses attended

SIMM05, IMDL03, ITSA02, GEEE01 and MOVE07

Testimonials

PRStats offers a great lineup of courses on statistical and analytical methods that are super relevant for ecologists and biologists. My lab and I have taken several of their courses—like Bayesian mixing models, time series analysis, and machine/deep learning—and we've found them very informative and directly useful for our work. I often recommend PRStats to my students and colleagues as a great way to brush up on or learn new R-based statistical skills.

Rolando O. Santos

PhD Assistant Professor, Florida International University

Courses attended

SIMM05, IMDL03, ITSA02, GEEE01 and MOVE07

Frequently asked questions

Everything you need to know about the product and billing.

When will I receive instructions on how to join?

You’ll receive an email on the Friday before the course begins, with full instructions on how to join via Zoom. Please ensure you have Zoom installed in advance.

Do I need administrator rights on my computer?

Yes — administrator access is recommended, as you may need to install software during the course. If you don’t have admin rights, please contact us before the course begins and we’ll provide a list of software to install manually.

I’m attending the course live — will I also get access to the session recordings?

Yes. All participants will receive access to the recordings for 30 days after the course ends.

I can’t attend every live session — can I join some sessions live and catch up on others later?

Absolutely. You’re welcome to join the live sessions you can and use the recordings for those you miss. We do encourage attending live if possible, as it gives you the chance to ask questions and interact with the instructor. You’re also welcome to send questions by email after the sessions.

I’m in a different time zone and plan to follow the course via recordings. When will these be available?

We aim to upload recordings on the same day, but occasionally they may be available the following day.

I can’t attend live — how can I ask questions?

You can email the instructor with any questions. For more complex topics, we’re happy to arrange a short Zoom call at a time that works for both of you.

Will I receive a certificate?

Yes. All participants receive a digital certificate of attendance, which includes the course title, number of hours, course dates, and the instructor’s name.

When will I receive instructions on how to join?

You’ll receive an email on the Friday before the course begins, with full instructions on how to join via Zoom. Please ensure you have Zoom installed in advance.

Do I need administrator rights on my computer?

Yes — administrator access is recommended, as you may need to install software during the course. If you don’t have admin rights, please contact us before the course begins and we’ll provide a list of software to install manually.

I’m attending the course live — will I also get access to the session recordings?

Yes. All participants will receive access to the recordings for 30 days after the course ends.

I can’t attend every live session — can I join some sessions live and catch up on others later?

Absolutely. You’re welcome to join the live sessions you can and use the recordings for those you miss. We do encourage attending live if possible, as it gives you the chance to ask questions and interact with the instructor. You’re also welcome to send questions by email after the sessions.

I’m in a different time zone and plan to follow the course via recordings. When will these be available?

We aim to upload recordings on the same day, but occasionally they may be available the following day.

I can’t attend live — how can I ask questions?

You can email the instructor with any questions. For more complex topics, we’re happy to arrange a short Zoom call at a time that works for both of you.

Will I receive a certificate?

Yes. All participants receive a digital certificate of attendance, which includes the course title, number of hours, course dates, and the instructor’s name.

Still have questions?

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RNAA01 ONLINE
RNAA01 ONLINE
£ 350.00
Unlimited
£350.00
26th January 2026 - 29th January 2026
Delivered remotely (United Kingdom), Western European Time Zone, United Kingdom
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